A549 cells were subjected to SDS PAGE followed by western blot with FNab08209(SQLE antibody) at dilution of 1:300
IP Result of anti-SQLE (IP:FNab08209, 3ug; Detection:FNab08209 1:300) with A549 cells lysate 920ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab08209(SQLE Antibody) at dilution of 1:50
Background
SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation.(PMID:21356516) The MW of SQLE is about 64kd.